Article
Detection of hepatitis B pre-core mutant by allele specific polymerase chain reaction.
Journal of clinical pathology - 1 Aug 1992
Lo E S, Lo Y M, Tse C H, Fleming K A
Abstract excerpt
AIM: Development of a specific polymerase chain reaction (PCR) assay for detection of the pre-core, stop codon, mutant of hepatitis B virus (HBV). METHODS: PCR primers, specific at the 3'-end for nucleotide 1896 of either the pre-core, stop codon, mutant or wild type HBV, were synthesised using published sequence data. Positive control templates for both types of virus were synthesised by the PCR, incorporating...
Topics
- Alleles
- Base Sequence
- DNA, Viral
- Hepatitis B
- Hepatitis B virus
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Species Specificity