Article
Detection of hepatitis B precore mutants by the fluorescent linear polymerase chain reaction sequencing method.
Journal of hepatology - 1 Dec 1994
Rodriguez-Frias F, Buti M, Arranz J A, Cotrina M, Jardi R, Esteban R, Guardia J
Abstract excerpt
To evaluate the presence of precore mutants, serum samples from 25 patients with chronic hepatitis B, HBV-DNA positive (5 HBeAg and 20 anti-HBe positive) were studied. The complete precore-core region of HBV-DNA was directly sequenced after polymerase chain reaction amplification by a fluorescent...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA, Viral
- Hepatitis Antibodies
- Hepatitis B
- Hepatitis B Core Antigens
- Hepatitis B e Antigens
- Humans
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Polymerase Chain Reaction