Article
[Barnase mutant Ser57Ala: preparation and properties].
Molekuliarnaia biologiia - 1 Jan 2000
Kolbanovskaia E Iu, Okorokov A L, Panov K I, Poliakov K M, Hartley R W, Karpeĭskiĭ M Ia
Abstract excerpt
Barnase, an extracellular ribonuclease produced by Bacillus amyloliquefaciens, belongs to a family of small microbial ribonucleases with similar structure and properties. These enzymes hydrolyze phosphodiester bonds on the 3' side of guanosine nucleotides in RNA. The guanylic specificity of barnase is more pronounced in the hydrolysis of dinucleotides or cyclonucleotide phosphates as substrates than in the...
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