[Barnase mutant Ser57Ala: preparation and properties].

Article

[Barnase mutant Ser57Ala: preparation and properties].

Molekuliarnaia biologiia - 1 Jan 2000

Kolbanovskaia E Iu, Okorokov A L, Panov K I, Poliakov K M, Hartley R W, Karpeĭskiĭ M Ia

Abstract excerpt

Barnase, an extracellular ribonuclease produced by Bacillus amyloliquefaciens, belongs to a family of small microbial ribonucleases with similar structure and properties. These enzymes hydrolyze phosphodiester bonds on the 3' side of guanosine nucleotides in RNA. The guanylic specificity of barna...

Topics

Alanine
Amino Acid Sequence
Bacterial Proteins
Base Sequence
Hydrolysis
Kinetics
Molecular Sequence Data
Mutation
Protein Conformation
Ribonucleases
Serine
Substrate Specificity

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