Article
[Mutations in the alpha-amylase gene of Bacillus amyloliquefaciens, leading to a decrease in the temperature of protein inactivation].
Molekuliarnaia biologiia - 1 Jan 2000
Smirnova N A, Sorokin A V, Iomantas Iu V, Abalakina E G, Kozlov Iu I
Abstract excerpt
Alpha-amylase genes of Bacillus amyloliquefaciens, coding proteins with reduced thermostability, had been obtained as a result of hydroxylamine mutagenesis. Temperature, pH and starch concentration dependences of two mutant alpha-amylases were investigated. The synthesis of the alpha-amylases by several B. subtilis strains with different levels of extracellular proteases was also studied. The mutation containing...
Topics
- Bacillus
- Enzyme Stability
- Genes, Bacterial
- Hot Temperature
- Hydrogen-Ion Concentration
- Mutation
- Plasmids
- Restriction Mapping
- alpha-Amylases