Article
[Two-codon mutagenesis of alpha-amylase gene of Bacillus amyloliquefaciens].
Molekuliarnaia biologiia - 1 Jan 2000
Smirnova N A, Sorokin A V, Laptev D A, Veĭko V P, Kozlov Iu I
Abstract excerpt
The oligonucleotide encoding Bam HI recognition site having the structure pCGGGATC had been inserted into the recognition sites MspI of the B. amyloliquefaciens alpha-amylase gene, which was cloned in pTG29B plasmid. The alpha-amylase gene had no BamHI sites before mutagenesis. The set of pNSBamH...
Topics
- Bacillus
- Codon
- Deoxyribonuclease HpaII
- Deoxyribonucleases, Type II Site-Specific
- Enzyme Stability
- Genes, Bacterial
- Hot Temperature
- Mutation
- Plasmids
- RNA, Messenger
- Restriction Mapping
- alpha-Amylases