Article
[Two-codon mutagenesis of alpha-amylase gene of Bacillus amyloliquefaciens].
Molekuliarnaia biologiia - 1 Jan 2000
Smirnova N A, Sorokin A V, Laptev D A, Veĭko V P, Kozlov Iu I
Abstract excerpt
The oligonucleotide encoding Bam HI recognition site having the structure pCGGGATC had been inserted into the recognition sites MspI of the B. amyloliquefaciens alpha-amylase gene, which was cloned in pTG29B plasmid. The alpha-amylase gene had no BamHI sites before mutagenesis. The set of pNSBamHI plasmids with BamHI site at four different positions was obtained. It was shown that all the mutant alpha-amylases...
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