Article
Isolation and characterization of mutants of Tus, the replication arrest protein of Escherichia coli.
The Journal of biological chemistry - 12 Aug 1994
Skokotas A, Wrobleski M, Hill T M
Abstract excerpt
Mutations in the tus gene of Escherichia coli, which encodes the replication arrest protein Tus, were isolated using a selection scheme based on the plasmid pHV750T2+, which transforms tus mutants at a much higher frequency than wild type strains. Seven mutants containing single nucleotide substi...
Topics
- Bacterial Proteins
- Base Sequence
- Catalysis
- Chymotrypsin
- DNA Helicases
- DNA Primers
- Escherichia coli
- Escherichia coli Proteins
- Kinetics
- Molecular Sequence Data
- Mutagenesis
- Mutation