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Article

The Mutant βE202K Sliding Clamp Protein Impairs DNA Polymerase III Replication Activity.

Journal of bacteriology - 5 Nov 2021

Homiski Caleb, Scotland Michelle K, Babu Vignesh M P, Chodavarapu Sundari, Maul Robert W, Kaguni Jon M, Sutton Mark D

Abstract excerpt

Expression of the Escherichia coli dnaN-encoded β clamp at ≥10-fold higher than chromosomally expressed levels impedes growth by interfering with DNA replication. We hypothesized that the excess β clamp sequesters the replicative DNA polymerase III (Pol III) to inhibit replication. As a test of this hypothesis, we obtained eight mutant clamps with an inability to impede growth and measured their ability to...

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Identifiers

PMID: 34543108
DOI: 10.1128/JB.00303-21
PMCID: PMC8570274

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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