Article
Mutation and altered expression of p53 genes in experimental rat bladder tumor cells.
Molecular carcinogenesis - 1 Feb 1994
Jones R F, Matuszyk J, Debiec-Rychter M, Wang C Y
Abstract excerpt
p53 genes were analyzed for mutations and expression in a series of 24 tumors or hyperplastic lesions of the urinary bladder induced in F344 rats by carcinogen treatment. Of these, 18 were analyzed as short-term urothelial cultures. Polymerase chain reaction-single-strand conformation polymorphism analysis and DNA sequencing were used to detect alterations in p53 genes or cDNAs, and the relative amounts of p53...
Topics
- Animals
- Base Sequence
- Blotting, Northern
- Carcinogens
- Exons
- Gene Expression
- Genes, p53
- Humans
- Molecular Sequence Data
- Mutation
- Neoplasms, Experimental
- Polymerase Chain Reaction