Article
p53 mutations in human bladder cancer.
Environmental and molecular mutagenesis - 1 Jan 1994
Kusser W C, Miao X, Glickman B W, Friedland J M, Rothman N, Hemstreet G P, Mellot J, Swan D C, Schulte P A, Hayes R B
Abstract excerpt
Mutations in the tumor suppressor gene p53 play an important role in carcinogenesis and tumor progression. To assess the status of p53 from genomic DNA from bladder cancer samples a two stage polymerase chain reaction was employed. The technique provided material for subsequent detection of mutat...
Topics
- Autoradiography
- Base Sequence
- Carcinoma in Situ
- Carcinoma, Transitional Cell
- DNA, Neoplasm
- DNA, Single-Stranded
- Electrophoresis, Agar Gel
- Exons
- Genes, p53
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Smoking
- Spectrometry, Fluorescence