Article
Isolation and characterization of mutant CHO cell lines with compartment-specific resistance to brefeldin A.
The Journal of cell biology - 1 Jul 1994
Yan J P, Colon M E, Beebe L A, Melançon P
Abstract excerpt
22 CHOBFY (BFY) cell lines were isolated at a frequency 2-30 x 10(-7) from mutagenized populations on the basis of their ability to grow in the presence of 1 microgram/ml brefeldin A (BFA). Four of the five mutant lines tested are genetically stable and none of the mutant lines characterized degrade this drug. Immunofluorescence studies reveal that whereas early endosomes and the Golgi complex have nearly...
Topics
- ADP-Ribosylation Factors
- Animals
- Brefeldin A
- CHO Cells
- Cell Compartmentation
- Cell Division
- Coatomer Protein
- Cricetinae
- Cyclopentanes
- Dose-Response Relationship, Drug
- Drug Resistance