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Article

DS28-6, a temperature-sensitive mutant of Chinese hamster ovary cells, expresses key phenotypic changes associated with brefeldin A treatment.

Proceedings of the National Academy of Sciences of the United States of America - 1 Nov 1991

Zuber C, Roth J, Misteli T, Nakano A, Moremen K

Abstract excerpt

The temperature-sensitive Chinese hamster ovary (CHO) cell mutant DS28-6 has been previously shown to be pleiotropically defective in protein secretion. We have examined the mutant cells to determine the intracellular site of the block in secretion. By transmission electron microscopy a time-dependent disassembly of the Golgi apparatus was found under nonpermissive temperature, which resulted in the loss of the...

Topics

Animals
Biological Transport
Brefeldin A
CHO Cells
Cricetinae
Cyclopentanes
Endoplasmic Reticulum
Fluorescent Antibody Technique
Galactosyltransferases
Glycosylation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Golgi Apparatus

Intracellular Membranes

Microscopy, Electron

Mutation

Phenotype

Protein Processing, Post-Translational

Proteins

Temperature

Source

Open in PubMed Open full text

Identifiers

PMID: 1946407
DOI: 10.1073/pnas.88.21.9818
PMCID: PMC52812

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1946407-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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DS28-6, a temperature-sensitive mutant of Chinese hamster ovary cells, expresses key phenotypic changes associated with brefeldin A treatment.PMID 1946407Read ↗

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