Article
ES-cells carrying two inactivated myf-5 alleles form skeletal muscle cells: activation of an alternative myf-5-independent differentiation pathway.
Developmental biology - 1 Jul 1994
Braun T, Arnold H H
Abstract excerpt
Both alleles of the myogenic regulatory gene myf-5 have been inactivated in mouse embryonic stem cells by different strategies involving either consecutive gene targeting with neomycin and hygromycin replacement vectors or spontaneous loss of heterozygosity in cells targeted with the neomycin replacement vector alone. Both selection schemes provided homozygous myf-5 mutant ES-cells with normal developmental...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.