ES-cells carrying two inactivated myf-5 alleles form skeletal muscle cells: activation of an alternative myf-5-independent differentiation pathway.

doi:10.1006/dbio.1994.1177

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Article

ES-cells carrying two inactivated myf-5 alleles form skeletal muscle cells: activation of an alternative myf-5-independent differentiation pathway.

Developmental biology - 1 Jul 1994

Braun T, Arnold H H

Abstract excerpt

Both alleles of the myogenic regulatory gene myf-5 have been inactivated in mouse embryonic stem cells by different strategies involving either consecutive gene targeting with neomycin and hygromycin replacement vectors or spontaneous loss of heterozygosity in cells targeted with the neomycin rep...

Topics

Alleles
Animals
Cell Differentiation
Cells, Cultured
DNA-Binding Proteins
Embryo, Mammalian
Lac Operon
Mice
Muscle Proteins
Muscles
Mutation
MyoD Protein
Myogenic Regulatory Factor 5
Myogenin
Stem Cells
Trans-Activators

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