Article
Genotyping compared with protein phenotyping of the common apolipoprotein E polymorphism.
Clinica chimica acta; international journal of clinical chemistry - 31 Jan 1994
Hansen P S, Gerdes L U, Klausen I C, Gregersen N, Faergeman O
Abstract excerpt
Apolipoprotein E (apo E) genotypes have been determined in 460 Danish men, with the use of the polymerase chain reaction (PCR) to amplify a 244 base pair fragment spanning the first-base polymorphic sites in the codons of amino acids 112 and 158 followed by restriction endonuclease cleavage. The results were compared with the apo E phenotypes previously determined by isoelectric focusing (IEF) of delipidated...
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