Article
Apolipoprotein E polymorphism determined by restriction enzyme analysis of DNA amplified by polymerase chain reaction: convenient alternative to phenotyping by isoelectric focusing.
Clinical chemistry - 1 Dec 1990
Kontula K, Aalto-Setälä K, Kuusi T, Hämäläinen L, Syvänen A C
Abstract excerpt
Three common alleles determine six apolipoprotein E (apo E) phenotypes that are associated with variations in serum cholesterol in the population. This genetic variation results from single nucleotide alterations at two DNA loci encoding the amino acid residues 112 and 158 of apo E. We compared r...
Topics
- Adolescent
- Adult
- Alleles
- Apolipoproteins E
- Base Sequence
- Cholesterol
- DNA
- Electrophoresis, Gel, Two-Dimensional
- Female
- Genotype
- Humans
- Isoelectric Focusing
- Male
- Middle Aged
- Molecular Sequence Data
- Phenotype
- Polymerase Chain Reaction