Article
Activation of a GTP-binding protein and a GTP-binding-protein-coupled receptor kinase (beta-adrenergic-receptor kinase-1) by a muscarinic receptor m2 mutant lacking phosphorylation sites.
European journal of biochemistry - 1 Dec 1994
Kameyama K, Haga K, Haga T, Moro O, Sadée W
Abstract excerpt
A mutant of the human muscarinic acetylcholine receptor m2 subtype (m2 receptor), lacking a large part of the third intracellular loop, was expressed and purified using the baculovirus/insect cell culture system. The mutant was not phosphorylated by beta-adrenergic-receptor kinase, as expected fr...
Topics
- Animals
- Baculoviridae
- Binding Sites
- Carbachol
- Cattle
- Cell Line
- Cyclic AMP-Dependent Protein Kinases
- Enzyme Activation
- GTP-Binding Proteins
- Guanosine 5'-O-(3-Thiotriphosphate)
- Guanosine Diphosphate
- Humans
- Mutation
- Phosphorylation
- Quinuclidinyl Benzilate