Article
A mutation of the beta 2-adrenergic receptor impairs agonist activation of adenylyl cyclase without affecting high affinity agonist binding. Distinct molecular determinants of the receptor are involved in physical coupling to and functional activation of Gs.
The Journal of biological chemistry - 25 Jan 1990
Hausdorff W P, Hnatowich M, O'Dowd B F, Caron M G, Lefkowitz R J
Abstract excerpt
Activation of guanyl nucleotide regulatory proteins (G proteins) by hormones and neurotransmitters appears to require the formation of high affinity agonist-receptor-G protein ternary complexes. In the case of the beta 2-adrenergic receptor, multiple regions of the molecule have been implicated i...
Topics
- Adenylyl Cyclases
- Amino Acid Sequence
- Cell Compartmentation
- DNA Mutational Analysis
- Endocytosis
- Enzyme Activation
- GTP-Binding Proteins
- Guanylyl Imidodiphosphate
- Humans
- In Vitro Techniques
- Isoproterenol
- Macromolecular Substances
- Molecular Sequence Data