Functional analysis of a dominant negative mutant of G alpha i2.

Abstract excerpt

The key event in receptor-catalyzed activation of heterotrimer G proteins is binding of GTP, which leads to subunit dissociation generating GTP-bound alpha subunits and free beta gamma complexes. We have previously identified a mutation that abolished GTP binding in G alpha o (S47C) and demonstrated that the mutant retained the ability to bind beta gamma and could act in a dominant negative fashion when expressed...

Topics

• Adenosine Diphosphate Ribose
• Animals
• Cell Line
• Enzyme Activation
• GTP-Binding Proteins
• Genes, Dominant
• Mutation
• Pertussis Toxin
• Signal Transduction
• Type C Phospholipases
• Virulence Factors, Bordetella