Article
Sequestration of muscarinic acetylcholine receptor m2 subtypes. Facilitation by G protein-coupled receptor kinase (GRK2) and attenuation by a dominant-negative mutant of GRK2.
The Journal of biological chemistry - 23 Dec 1994
Tsuga H, Kameyama K, Haga T, Kurose H, Nagao T
Abstract excerpt
Sequestration of m2 receptors (muscarinic acetylcholine receptor m2 subtypes), which was assessed as loss of N-[3H]methylscopolamine ([3H]NMS) binding activity from the cell surface, was examined in COS 7 and BHK-21 cells that had been transfected with expression vectors encoding the m2 receptor and, independently, vectors encoding a G protein-coupled receptor kinase (GRK2) (beta-adrenergic receptor kinase 1) or...
Topics
- Animals
- Base Sequence
- Carbachol
- Cells, Cultured
- Chlorocebus aethiops
- Cricetinae
- Cyclic AMP-Dependent Protein Kinases
- DNA Primers
- GTP-Binding Proteins
- Genes, Dominant