Analysis of NS3-mediated processing of the hepatitis C virus non-structural region in vitro.

doi:10.1099/0022-1317-75-12-3469

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Article

Analysis of NS3-mediated processing of the hepatitis C virus non-structural region in vitro.

The Journal of general virology - 1 Dec 1994

D'Souza E D, O'Sullivan E, Amphlett E M, Rowlands D J, Sangar D V, Clarke B E

Abstract excerpt

The protease activity of the hepatitis C virus (HCV) NS3 protein has been investigated using transient expression methods in mammalian cells, as well as in vitro transcription/translation systems. We confirmed that expression of the NS3-5 polyprotein in rabbit reticulocyte lysates results in effi...

Topics

Animals
Base Sequence
Binding Sites
Cell-Free System
Cloning, Molecular
Hepacivirus
Humans
Molecular Sequence Data
Mutation
Protein Biosynthesis
Protein Precursors
Protein Processing, Post-Translational

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Rabbits

Recombinant Proteins

Sequence Alignment

Serine Endopeptidases

Transcription, Genetic

United Kingdom

Source

Open in PubMed

Identifiers

PMID: 7996139
DOI: 10.1099/0022-1317-75-12-3469

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7996139-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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