Article
Proteolytic processing of a Murray Valley encephalitis virus non-structural polyprotein segment containing the viral proteinase: accumulation of a NS3-4A precursor which requires mature NS3 for efficient processing.
The Journal of general virology - 1 Sept 1992
Lobigs M
Abstract excerpt
The proteolytic processing of a non-structural polyprotein segment from the cytoplasmic domain of NS2A to the C terminus of NS5 of Murray Valley encephalitis (MVE) virus was examined, when expressed from cDNA via a vaccinia virus recombinant, in transiently transfected COS cells, or synthesized by cell-free translation. Cleavages mediated by the virus-encoded proteinase domain in NS3 at the junctions of NS2A-2B,...
Topics
- Animals
- Base Sequence
- Cell-Free System
- Cells, Cultured
- DNA, Single-Stranded
- Endopeptidases
- Flavivirus
- Molecular Sequence Data
- Mutation
- Protein Precursors
- Protein Processing, Post-Translational