Use of stable dye-DNA intercalating complexes to detect cystic fibrosis mutations.

doi:10.1006/mcpr.1994.1035

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Article

Use of stable dye-DNA intercalating complexes to detect cystic fibrosis mutations.

Molecular and cellular probes - 1 Jun 1994

Axton R A, Brock D J

Abstract excerpt

Several dyes are now known which intercalate into double-stranded DNA with fluorescence enhancement. One such is TOTO (1,1'-(4,4,7,7-tetramethyl-4,7-diazaundecamethylene)-bis-4-[3-meth yl-2,3- dihydro-(benzo-1,3-thiazole)-2-methlyidene]-quinolinium tetraiodide), a homodimer of thiazole orange, wh...

Topics

Alleles
Base Sequence
Cystic Fibrosis
DNA
Fluorescence
Heterozygote
Humans
Mass Screening
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Protein Binding
Quinolinium Compounds
Thiazoles

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