Detection of multiple cystic fibrosis mutations by reverse dot blot hybridization: a technology for carrier screening.

Abstract excerpt

We describe the implementation of a modified version of the reverse dot blot hybridization technology to detect eight cystic fibrosis mutations. The method is simple, quick, reliable, inexpensive, and nonradioactive and utilizes the sensitivity of the polymerase chain reaction coupled with colore...

Topics

• Base Sequence
• Cystic Fibrosis
• Genetic Carrier Screening
• Genetic Testing
• Humans
• Molecular Sequence Data
• Mutation
• Oligodeoxyribonucleotides
• Polymerase Chain Reaction
• Thalassemia