Article
Minisequencing with acyclonucleoside triphosphates tethered to lanthanide(III) chelates.
Bioconjugate chemistry - 1 Jun 2008
Ollikka Pia, Ylikoski Alice, Kaatrasalo Annukka, Harvala Heli, Hakala Harri, Hovinen Jari
Abstract excerpt
Four acyclic nucleoside triphosphates (derivatives of cytosine, thymine, 7-deazaadenine, and 7-deazaguanine) labeled with nonluminescent europium, terbium, dysprosium, and samarium chelates of 2,2',2'',2'''-[[4-(4-isothiocyanatophenyl)ethyl]pyridine-2,6-diyl]bis(methylenenitrilo)]tetrakis(acetic acid) were applied to minisequencing using two mutations (Delta F 508 and 1717-1 G to A) of cystic fibrosis as a model...
Topics
- Base Sequence
- Chelating Agents
- Cystic Fibrosis Transmembrane Conductance Regulator
- Genotype
- Lanthanoid Series Elements
- Molecular Sequence Data
- Nucleotides
- Oligonucleotides
- Point Mutation
- Polymerase Chain Reaction