Article
Trans-acting transposase mutant from Tn5.
Proceedings of the National Academy of Sciences of the United States of America - 15 Jul 1991
DeLong A, Syvanen M
Abstract excerpt
Transposition of Tn5 and of its component insertion sequence IS50R is regulated through the action of two proteins it encodes: a cis-acting transposase, Tnp, and a trans-acting inhibitor of transposition, Inh. The mechanism of the cis-acting Tnp and the relevance of inhibition to cis action have been addressed in the current study. A specific colony morphology assay for transposition of Tn5 was shown to be...
Topics
- Bacteriophage lambda
- DNA Transposable Elements
- Escherichia coli
- Genotype
- Mutation
- Nucleotidyltransferases
- Phenotype
- Plasmids
- Restriction Mapping
- Transposases