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Article

Characterization of two hypertransposing Tn5 mutants.

Journal of bacteriology - 1 Feb 1992

Wiegand T W, Reznikoff W S

Abstract excerpt

Transposition of Tn5 in Escherichia coli is regulated by two transposon-encoded proteins: transposase (Tnp), promoting transposition preferentially in cis, and the trans-acting inhibitor (Inh). Two separate transposase mutants were isolated that replace glutamate with lysine at position 110 (EK110) and at position 345 (EK345). The EK transposase proteins increase the Tn5 transposition frequency 6- to 16-fold in...

Topics

Base Sequence
DNA Transposable Elements
DNA, Bacterial
Escherichia coli
Genes, Regulator
Immunoblotting
Molecular Sequence Data
Mutation
Nucleotidyltransferases
Transposases

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Identifiers

PMID: 1310499
DOI: 10.1128/jb.174.4.1229-1239.1992
PMCID: PMC206416

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1310499-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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