Article
Hyperactive transposase mutants of the Himar1 mariner transposon.
Proceedings of the National Academy of Sciences of the United States of America - 28 Sept 1999
Lampe D J, Akerley B J, Rubin E J, Mekalanos J J, Robertson H M
Abstract excerpt
Mariner-family transposable elements are active in a wide variety of organisms and are becoming increasingly important genetic tools in species lacking sophisticated genetics. The Himar1 element, isolated from the horn fly, Haematobia irritans, is active in Escherichia coli when expressed appropriately. We used this fact to devise a genetic screen for hyperactive mutants of Himar1 transposase that enhance overall...
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