Article
HLA-DQA1 allele and suballele typing using noncoding sequence polymorphisms. Application to 4AOHW cell panel typing.
Human immunology - 1 Sept 1993
Limm T M, Ashdown M L, Naughton M J, McGinnis M D, Simons M J
Abstract excerpt
HLA-DQA1 typing of the 4AOHW cell panel is presented using a novel strategy that exploits both intron and exon polymorphisms. Intron sequences adjacent to the variable HLA-DQA1 second exon exhibit stable polymorphisms that are specific for locus alleles and certain suballelic DR/DQ haplotypes. A...
Topics
- Alleles
- Base Sequence
- Cell Line, Transformed
- Deoxyribonucleases, Type II Site-Specific
- Exons
- HLA-DQ Antigens
- HLA-DQ alpha-Chains
- HLA-DR Antigens
- Haplotypes
- Histocompatibility Testing
- Humans
- Introns
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length