Article
A simple and rapid method for HLA-DP genotyping by digestion of PCR-amplified DNA with allele-specific restriction endonucleases.
Human immunology - 1 Feb 1990
Maeda M, Uryu N, Murayama N, Ishii H, Ota M, Tsuji K, Inoko H
Abstract excerpt
We previously reported a simple and rapid method for HLA-DQA genotyping by digestion of polymerase chain reaction-amplified DQA genes with allele-specific restriction endonucleases. Here we report the application of this method to DP genotyping. The second exon of the HLA-DPB genes was selectively amplified from genomic DNAs of 72 HLA-D homozygous B-cell lines by the polymerase chain reaction method. Amplified...
Topics
- Alleles
- Base Sequence
- DNA
- DNA Restriction Enzymes
- Genotype
- HLA-DP Antigens
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Restriction Mapping