Both sphingolipids and cholesterol participate in the detergent insolubility of alkaline phosphatase, a glycosylphosphatidylinositol-anchored protein, in mammalian membranes.

doi:10.1074/jbc.270.11.6254

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Article

Both sphingolipids and cholesterol participate in the detergent insolubility of alkaline phosphatase, a glycosylphosphatidylinositol-anchored protein, in mammalian membranes.

The Journal of biological chemistry - 17 Mar 1995

Hanada K, Nishijima M, Akamatsu Y, Pagano R E

Abstract excerpt

SPB-1, a Chinese hamster ovary cell variant defective in serine palmitoyltransferase activity for sphingolipid synthesis, provides a useful system for studying the effects of sphingolipids and/or cholesterol deprivation on cellular functions and membrane properties. To investigate whether there was an interaction among sphingolipids, cholesterol, and glycosylphosphatidylinositol (GPI)-anchored proteins in...

Topics

Acyltransferases
Alkaline Phosphatase
Animals
Blotting, Western
CHO Cells
Caveolin 1
Caveolins
Cell Membrane
Cholesterol
Cricetinae
Detergents

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Female

Genetic Variation

Glycosylphosphatidylinositols

Humans

Membrane Proteins

Microscopy, Electron

Phosphatidylinositol Diacylglycerol-Lyase

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Open in PubMed

Identifiers

PMID: 7890763
DOI: 10.1074/jbc.270.11.6254

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7890763-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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