Article
Sphingolipids are essential for the growth of Chinese hamster ovary cells. Restoration of the growth of a mutant defective in sphingoid base biosynthesis by exogenous sphingolipids.
The Journal of biological chemistry - 25 Nov 1992
Hanada K, Nishijima M, Kiso M, Hasegawa A, Fujita S, Ogawa T, Akamatsu Y
Abstract excerpt
We previously isolated a temperature-sensitive Chinese hamster ovary cell mutant (strain SPB-1) with thermolabile serine palmitoyltransferase, which is involved in the first step of sphingolipid synthesis (Hanada, K., Nishijima, M., and Akamatsu, Y. (1990) J. Biol. Chem. 265, 22137-22142). In this study, sphingolipid-deficient culture medium was used to examine the effect of exogenous sphingolipids on the cell...
Topics
- Acyltransferases
- Animals
- CHO Cells
- Cell Division
- Cell Survival
- Cricetinae
- Genetic Variation
- Kinetics
- Mutation
- Serine C-Palmitoyltransferase
- Sphingolipids