Article
A temperature-sensitive mammalian cell mutant with thermolabile serine palmitoyltransferase for the sphingolipid biosynthesis.
The Journal of biological chemistry - 25 Dec 1990
Hanada K, Nishijima M, Akamatsu Y
Abstract excerpt
We devised an in situ assay method for the activity of serine palmitoyltransferase (SPT) that catalyzes the first step in sphingolipid biosynthesis and isolated a temperature-sensitive mutant of Chinese hamster ovary cells with thermolabile SPT. This mutant stopped growing at 40 degrees C after s...
Topics
- Acyltransferases
- Animals
- Cell Division
- Cell Line
- Cricetinae
- Cricetulus
- Female
- Kinetics
- Mutation
- Ovary
- Phospholipids
- Serine
- Serine C-Palmitoyltransferase
- Sphingolipids
- Temperature