Article
Robust nonradioactive oligonucleotide ligation assay to detect a common point mutation in the CYP2D6 gene causing abnormal drug metabolism.
Clinical chemistry - 1 Mar 1995
Hansen T S, Petersen N E, Iitiä A, Blaabjerg O, Hyltoft-Petersen P, Hørder M
Abstract excerpt
A new nonradioactive oligonucleotide ligation assay for the detection of a common point mutation in the CYP2D6 gene is presented. The assay takes advantage of simultaneous time-resolved fluorescence measurements of lanthanide-labeled probes and the specificity of T4-DNA ligase in combination with...
Topics
- Adenosine Triphosphate
- Cytochrome P-450 CYP2D6
- Cytochrome P-450 Enzyme System
- DNA Ligases
- DNA Mutational Analysis
- Europium
- Genotype
- Humans
- Mixed Function Oxygenases
- Oligonucleotides
- Pharmaceutical Preparations
- Point Mutation
- Polymerase Chain Reaction
- Samarium
- Sodium Chloride
- Temperature