Frequent deletions and sequence aberrations at the transgene junctions of transgenic mice carrying the papillomavirus regulatory and the SV40 TAg gene sequences.

Abstract excerpt

Exogenous DNA microinjected into one-cell mouse zygotes either integrates into the host genome within a short time span, or is rapidly degraded. On integration, a transgene sequence is frequently reiterated. In this report, we describe the enzymatic amplification analysis of transgene junctions of 12 transgenic mice carrying different copy numbers of the same transgene with dissimilar ends. The transgene was...

Topics

• Animals
• Antigens, Polyomavirus Transforming
• Base Sequence
• DNA Primers
• Gene Deletion
• Mice
• Mice, Transgenic
• Molecular Sequence Data
• Mutation
• Papillomaviridae
• Polymerase Chain Reaction