Article
Transgene insertion pattern analysis using genomic walking in a transgenic mouse line.
Experimental animals - 1 Jan 2006
Suzuki Osamu, Hata Tomoko, Takekawa Naho, Koura Minako, Takano Kaoru, Yamamoto Yoshie, Noguchi Yoko, Uchio-Yamada Kozue, Matsuda Junichiro
Abstract excerpt
A transgene mapping technique (Noguchi et al., Exp. Anim. 53:103-111, 2004) is described that can be used to analyze transgene integration patterns in transgenic mice. The technique was used to reveal that a transgenic mouse line (GM1-sy#116) harbored inverted and direct tandem repeats of both intact and partial pCAGGS-based transgenes in the G2 region of chromosome 1. This complicated concatenation of transgenes...
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