Article
Molecular basis of IsK protein regulation by oxidation or chelation.
The Journal of biological chemistry - 24 Feb 1995
Busch A E, Waldegger S, Herzer T, Raber G, Gulbins E, Takumi T, Moriyoshi K, Nakanishi S, Lang F
Abstract excerpt
Slowly activating IsK channels were expressed in Xenopus oocytes and exposed to oxidative agents. Oxidative treatment reduced the resulting current IsK, while no inhibition was observed for IsK protein mutants carrying a Ser mutation instead of a highly conserved Cys residue in the intracellular domain. In contrast, Hg2+, which may not only oxidize thiol groups but also form chelates with dibasic amino acids,...
Topics
- Animals
- Chelating Agents
- Humans
- Mercury
- Mutation
- Oxidants
- Oxidation-Reduction
- Peroxides
- Potassium Channels
- Potassium Channels, Voltage-Gated
- Rats