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Article

Isolation and classification of temperature-sensitive mutants of influenza B virus.

Microbiology and immunology - 1 Jan 1994

Yamamoto-Goshima F, Aoki H, Takada T, Toda S, Yoshii S, Maeno K

Abstract excerpt

We isolated 25 temperature-sensitive mutants of B/Kanagawa/73 strain generated by mutagenesis with 5-fluorouracil and classified them into seven recombination groups by pair-wise crosses. All mutants showed a ratio of plaquing efficiency at the nonpermissive temperature (37.5 C) to the permissive temperature (32 C) of 10(-4) or less. At 37.5 C most of group I, II, and III mutants did not produce appreciable...

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Identifiers

PMID: 7854217
DOI: 10.1111/j.1348-0421.1994.tb01853.x

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