Article
An influenza A live attenuated reassortant virus possessing three temperature-sensitive mutations in the PB2 polymerase gene rapidly loses temperature sensitivity following replication in hamsters.
Vaccine - 1 Jan 2000
Murphy B R, Park E J, Gottlieb P, Subbarao K
Abstract excerpt
The purpose of the present study was to produce an influenza A H2N2 donor virus from which an attenuating PB2 gene bearing three discrete temperature sensitive (ts) mutations could be readily transferred to currently epidemic influenza A H1N1 and H3N2 viruses via genetic reassortment. An influenz...
Topics
- Animals
- Chickens
- Chlorocebus aethiops
- Cricetinae
- Female
- Influenza A virus
- Influenza Vaccines
- Mesocricetus
- Mutation
- RNA-Dependent RNA Polymerase
- Temperature
- Transfection