Article
Structure, stability and biological properties of a N-terminally truncated form of recombinant human interleukin-6 containing a single disulfide bond.
European journal of biochemistry - 15 Jan 1995
Breton J, La Fiura A, Bertolero F, Orsini G, Valsasina B, Ziliotto R, De Filippis V, Polverino de Laureto P, Fontana A
Abstract excerpt
A mutant species of the 185-residue chain of human interleukin-6 lacking 22-residues at its N-terminus and with a Cys-->Ser substitution at positions 45 and 51 was produced in Escherichia coli. The 163-residue protein des-(A1-S22)-[C45S, C51S]interleukin-6, containing a single disulfide bridge, f...
Topics
- Amino Acid Sequence
- Animals
- Cell Division
- Cell Line
- Disulfides
- Escherichia coli
- Humans
- Interleukin-6
- Mice
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Recombinant Proteins