The single mutation Phe173 --> Ala induces a molten globule-like state in murine interleukin-6.

doi:10.1021/bi991973i

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Article

The single mutation Phe173 --> Ala induces a molten globule-like state in murine interleukin-6.

Biochemistry - 29 Feb 2000

Matthews J M, Norton R S, Hammacher A, Simpson R J

Abstract excerpt

A series of three aromatic to alanine mutants of recombinant murine interleukin-6 lacking the 22 N-terminal residues (DeltaN22mIL-6) were constructed to investigate the role of these residues in the structure and function of mIL-6. While Y78A and Y97A have activities similar to that of DeltaN22mIL-6, F173A lacks biological activity. F173A retains high levels of secondary structure, as determined by far-UV...

Topics

Alanine
Animals
Cell Line
Circular Dichroism
Dose-Response Relationship, Drug
Hydrogen-Ion Concentration
Interleukin-6
Magnetic Resonance Spectroscopy
Mice
Models, Molecular
Mutation
Phenylalanine
Protein Conformation
Protein Folding
Protein Structure, Secondary
Protein Structure, Tertiary
Recombinant Proteins
Temperature

Source

Open in PubMed

Identifiers

PMID: 10684643
DOI: 10.1021/bi991973i

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