Article
Internal deletions of amino acids 29-42 of human interleukin-6 (IL-6) differentially affect bioactivity and folding.
FEBS letters - 19 Aug 1991
Arcone R, Fontaine V, Coto I, Brakenhoff J P, Content J, Ciliberto G
Abstract excerpt
Internal deletions of the human interleukin-6 (IL-6) cDNA have been generated in the region encoding residues 29 to 42. Mutant proteins were produced by in vitro transcription-translation or in Escherichia coli and tested for their biological activity using the hybridoma growth factor (HGF) assay...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Base Sequence
- Binding Sites
- Escherichia coli
- Humans
- Interleukin-6
- Molecular Sequence Data
- Mutation
- Peptide Fragments
- Precipitin Tests
- Protein Conformation
- Recombinant Proteins
- Tumor Cells, Cultured