Article
Structure-function analysis of human interleukin-6. Evidence for the involvement of the carboxy-terminus in function.
FEBS letters - 26 Mar 1990
Krüttgen A, Rose-John S, Möller C, Wroblowski B, Wollmer A, Müllberg J, Hirano T, Kishimoto T, Heinrich P C
Abstract excerpt
C-terminally deleted analogs of human interleukin-6 (IL-6) have been constructed at the cDNA level, and after cell-free transcription and translation their biological activity was analyzed. Removal of only 4 amino acids resulted in complete loss of biological activity as determined by the B9 cell...
Topics
- Animals
- Cell Line
- Circular Dichroism
- DNA
- Humans
- Interleukin-6
- Mutation
- Protein Biosynthesis
- Protein Conformation
- Rats
- Structure-Activity Relationship
- Transcription, Genetic