High-level expression of a biologically active human interleukin-6 mutein.

doi:10.1016/0168-1656(94)90168-6

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Article

High-level expression of a biologically active human interleukin-6 mutein.

Journal of biotechnology - 30 Apr 1994

Skelly S M, Tackney C, Hicklin D, Tamkins T, Goldstein N, Waksal H, Dagan S

Abstract excerpt

We have constructed two different muteins of interleukin-6 (IL-6) which were expressed in Escherichia coli. Both muteins lack the first 22 N-terminal amino acids of native IL-6 and lack one or the other of the two naturally occurring pairs of cysteines at either position 45 and 51 or position 74...

Topics

Animals
Humans
Interleukin-6
Mice
Mutation
Structure-Activity Relationship

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