Article
Binding of the bacteriophage T4 transcriptional activator, MotA, to T4 middle promoter DNA: evidence for both major and minor groove contacts.
Journal of molecular biology - 30 Jul 1999
Sharma M, Marshall P, Hinton D M
Abstract excerpt
During infection, the bacteriophage T4 transcriptional activator MotA, the co-activator AsiA, and host RNA polymerase are needed to transcribe from T4 middle promoters. Middle promoters contain a -10 region recognized by the sigma(70)subunit of RNA polymerase and a MotA box centered at -30 that is bound by MotA. We have investigated how the loss or modification of base determinants within the MotA box sequence...
Topics
- Bacteriophage T4
- Base Pairing
- Base Sequence
- Binding, Competitive
- Consensus Sequence
- DNA
- DNA Methylation
- DNA-Binding Proteins
- DNA-Directed RNA Polymerases
- Membrane Proteins
- Models, Genetic
- Mutation
- Promoter Regions, Genetic
- Response Elements
- Sigma Factor
- Trans-Activators
- Transcription Factors
- Transcription, Genetic