Article
Interaction of a group II intron ribonucleoprotein endonuclease with its DNA target site investigated by DNA footprinting and modification interference.
Journal of molecular biology - 1 Jun 2001
Singh N N, Lambowitz A M
Abstract excerpt
Group II intron mobility occurs by a target DNA-primed reverse transcription mechanism in which the intron RNA reverse splices directly into one strand of a double-stranded DNA target site, while the intron-encoded protein cleaves the opposite strand and uses it as a primer to reverse transcribe the inserted intron RNA. The group II intron endonuclease, which mediates this process, is an RNP particle that...
Topics
- Adenine
- Base Pairing
- Base Sequence
- Binding Sites
- DNA
- DNA Footprinting
- DNA Methylation
- DNA-Binding Proteins
- Deoxyribonuclease I
- Deoxyuridine
- Endonucleases
- Exons
- Introns
- Kinetics
- Lactococcus lactis