Article
AT base pairs are the main target for mutations at the hprt locus of rat skin fibroblasts exposed in vitro to the monofunctional alkylating agent N-ethyl-N-nitrosourea.
Mutagenesis - 1 Sept 1994
Jansen J G, van Teijlingen C M, Mohn G R, van Zeeland A A, Vrieling H
Abstract excerpt
Spectra of N-ethyl-N-nitrosourea (ENU)-induced mutations differ widely among various in vitro and in vivo mutational systems. To investigate possible reasons for these differences, a mutational system is needed in which the same target gene is used for comparison in the same type of cells in vitr...
Topics
- Alkylating Agents
- Amino Acid Sequence
- Animals
- Base Composition
- Base Sequence
- DNA
- Ethylnitrosourea
- Fibroblasts
- Hypoxanthine Phosphoribosyltransferase
- In Vitro Techniques
- Male
- Molecular Sequence Data
- Mutation
- Point Mutation
- Rats
- Rats, Wistar
- Skin