Article
DNA sequence analysis of spontaneous and N-ethyl-N-nitrosourea-induced hprt mutations arising in vivo in cynomolgus monkey T-lymphocytes.
Environmental and molecular mutagenesis - 1 Jan 1992
Harbach P R, Filipunas A L, Wang Y, Aaron C S
Abstract excerpt
The study of hprt mutations in cynomolgus monkey T-lymphocytes is part of our effort to understand the mechanisms of mutagenesis in vivo. This primate model allows us to study mutations and their kinetics at the molecular level under well-controlled conditions using recently developed techniques for selection of mutant T-cells and polymerase chain reaction (PCR) amplification of hprt cDNA, which is directly...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Codon
- DNA
- Ethylnitrosourea
- Hypoxanthine Phosphoribosyltransferase
- Macaca fascicularis
- Molecular Sequence Data
- Mutagenesis
- Mutation