Article
Escherichia coli tyrT gene transcription is sensitive to DNA supercoiling in its native chromosomal context: effect of DNA topoisomerase IV overexpression on tyrT promoter function.
Molecular microbiology - 1 Oct 1994
Free A, Dorman C J
Abstract excerpt
We have investigated the in vivo DNA supercoiling sensitivity of the Escherichia coli tRNA(1tyr) gene (tyrT) promoter in its normal chromosomal location. Here, the native tyrT promoter is found to be exquisitely sensitive to mutations and to drugs which alter the level of DNA supercoiling. We sho...
Topics
- Base Sequence
- DNA Topoisomerase IV
- DNA Topoisomerases, Type I
- DNA, Superhelical
- Escherichia coli
- Gene Expression
- Genes, Bacterial
- Genotype
- Molecular Sequence Data
- Mutagenesis, Insertional
- Oligodeoxyribonucleotides
- Open Reading Frames
- Phenotype
- Plasmids
- Promoter Regions, Genetic
- RNA, Transfer, Tyr
- Transcription, Genetic