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Article

Mechanism of transcriptional activation by FIS: role of core promoter structure and DNA topology.

Journal of molecular biology - 8 Aug 2003

Auner Helge, Buckle Malcolm, Deufel Annette, Kutateladze Tamara, Lazarus Linda, Mavathur Ramesh, Muskhelishvili Georgi, Pemberton Iain, Schneider Robert, Travers Andrew

Abstract excerpt

The Escherichia coli DNA architectural protein FIS activates transcription from stable RNA promoters on entry into exponential growth and also reduces the level of negative supercoiling. Here we show that such a reduction decreases the activity of the tyrT promoter but that activation by FIS rescues tyrT transcription at non-optimal superhelical densities. Additionally we show that three different "up" mutations...

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Identifiers

PMID: 12888342
DOI: 10.1016/s0022-2836(03)00727-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Mechanism of transcriptional activation by FIS: role of core promoter structure and DNA topology.PMID 12888342Read ↗

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