Article
Heterologous expression of the cytotoxin restriction in Aspergillus nidulans and Aspergillus niger.
Protein expression and purification - 1 Oct 1994
Brandhorst T, Yang R, Kenealy W R
Abstract excerpt
The cDNA clone of restrictocin was placed under the control of the glucoamylase promoter from Aspergillus awamori and was transformed into Aspergillus nidulans and Aspergillus niger. Site-specific changes were introduced into cDNA constructs and these were transformed into A. nidulans. The secretion signal sequence was deleted from one form of the gene and three mutations introduced single amino acid...
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