Heterologous expression of the cytotoxin restriction in Aspergillus nidulans and Aspergillus niger.

doi:10.1006/prep.1994.1068

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Article

Heterologous expression of the cytotoxin restriction in Aspergillus nidulans and Aspergillus niger.

Protein expression and purification - 1 Oct 1994

Brandhorst T, Yang R, Kenealy W R

Abstract excerpt

The cDNA clone of restrictocin was placed under the control of the glucoamylase promoter from Aspergillus awamori and was transformed into Aspergillus nidulans and Aspergillus niger. Site-specific changes were introduced into cDNA constructs and these were transformed into A. nidulans. The secretion signal sequence was deleted from one form of the gene and three mutations introduced single amino acid...

Topics

Allergens
Antigens, Plant
Aspergillus
Aspergillus nidulans
Aspergillus niger
Base Sequence
Blotting, Northern
Blotting, Southern
Cytotoxins
Fungal Proteins
Gene Expression Regulation, Fungal

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Glucan 1,4-alpha-Glucosidase

Molecular Sequence Data

Mutation

Plasmids

Promoter Regions, Genetic

Protein Synthesis Inhibitors

RNA, Messenger

Source

Open in PubMed

Identifiers

PMID: 7827506
DOI: 10.1006/prep.1994.1068

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7827506-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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