Display of Expression Products of cDNA Libraries on Phage Surfaces. A Versatile Screening System for Selective Isolation of Genes by Specific Gene-Product/Ligand Interaction

Abstract excerpt

Techniques for cloning cDNAs from bacteriophage libraries immobilised on solid supports are well established. However, these techniques do not allow selective enrichment of clones expressing proteins of interest. Screening of cDNA libraries would be simplified if the proteins encoded by cDNAs could be expressed on the surface of phage. Phage carrying genes encoding proteins for which a ligand is available can be...